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Single strand conformation polymorphism within butyrophilin gene and its relationship with milk yield in Indian riverine buffaloes |
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| รหัสดีโอไอ | |
| Creator | 1. Kale, D.S. 2. Yadav, B.R. 3. Anupama Mukherjee 4. Jagdish Prasad |
| Title | Single strand conformation polymorphism within butyrophilin gene and its relationship with milk yield in Indian riverine buffaloes |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 4 |
| Page no. | 253-259 |
| Keyword | Riverine buffaloes,Bubalus bubalis,Butyrophilin,DNA polymorphism,Riverine PCRSSCP,Milk yield,India |
| ISSN | 1256726 |
| Abstract | Butyrophilin has been shown to be a mammary-specifi c gene and used as a genetic marker to investigate allelic substitution effects on milk production traits. The aim of the present study was to study genetic polymorphism of the butyrophilin gene using SSCP in the Murrah, Surti and Bhadawari breeds of riverine buffaloes (Bubalus bubalis). The association study was carried out to fi nd possible relationships (if any) with milk production traits in Murrah buffalo. The SSCP analysis of the intron region of butyrophilin revealed polymorphic BTI1 SSCP in buffaloes. DNA sequencing of three polymorphic SSCPs within the Murrah buffalo butyrophilin gene revealed three variants.viz, A, B and C. The frequencies of identifi ed buffalo butyrophilin variants were: A = 0.6, B = 0.31 and C = 0.09 in 55 Murrah buffaloes, in Surti A = 0.5, B = 0.3 and C = 0.2 while in Bhadawari A= 0.4, 0.5 and 0.1. The Murrah buffalo butyrophilin variant sequence (EU194868) was found in 96 percent pairwise percent similarity with Bos taurus nucleotide sequence (AF005497). The statistical analysis using general linear model procedure (SYSTAT) for association study indicated BTI1 SSCP was signifi cantly associated (P LT= 0.05) with 305-day lactation milk yield of Murrah buffaloes. The Murrah buffaloes with BTI1BB genotypes had 683.93 kg and 320.48 kg higher milk yield as compared to BTI1AA and BTI1CC genotypes, respectively. The association of BTI1 SSCP polymorphism with milk production traits will be useful for genetic improvement of buffaloes for milk production traits. |
