Endocervical cytological studies in endometritis affected Murrah graded buffloes (Bubalus bubalis)
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Creator 1. Ramesh Babu, K.
2. Mouli Krishna, K.
3. Padmaja, K.
Title Endocervical cytological studies in endometritis affected Murrah graded buffloes (Bubalus bubalis)
Publisher International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand)
Publication Year 2556
Journal Title Buffalo Bulletin
Journal Vol. 32
Journal No. 3
Page no. 212-217
Keyword Murrah buffalo,Bubalus bubalis,Endometritis,Endometrial cytology,Infertility,India
ISSN 1256726
Abstract Objective of the study was to develop endometrial cytology as a tool to diagnose endometritis in field situations. A total of 65 cyclic Murrah graded buffaloes consisting of 25 normal healthy in Group 1 and 40 infertile in Group 2 were utilized. For cytological studies at day 0 and 4 of the cycle endocervical secretions by aspiration technique and lavage technique, respectively, were collected. At day 4 of the cycle endometrial biopsies were obtained for histopathological studies. In the present study, the aspiration at day 0 was successful in 100 percent attempts but cells were found 92.3 percent smears. The lavage at day 4 too was successful in 100 percent attempts and yielded cells in 96.93 percent smears. At two different sampling intervals both neutrophil count and lymphocyte count differed significantly (P LT= 0.05) between Groups 1 and 2. In Group 2, the neutrophil count differed signifi cantly between sampling intervals (P GT= 0.05), but not in Group 1. Lymphocyte count did not differ between sampling intervals in both groups, but differed significantly between groups. In the present study, based on histopathological lesions in Group 2 33, 28 and 39 percent biopsies were classified into acute, sub-acute and chronic endometritis, respectively. The mean neutrophil and lymphocyte counts did not differ between subgroups. Changes in the endometrium could be related to cytological findings at day 0 and 4 of the cycle. It was concluded that the cytological studies in genital secretions might conveniently be implemented in field situations.
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