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Evaluation of the superovulatory effect of pregnant mare's serum gonadotropin (PMSG) and follicle stimulating hormone (FSH) in the non-breeding season of river buffaloes of the Miankaleh Peninsula, northern Iran |
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| รหัสดีโอไอ | |
| Creator | 1. Behrang Ekrami 2. Hamid Ghasemzadeh-Nava 3. Parviz Tajik 4. Amin Tamadon |
| Title | Evaluation of the superovulatory effect of pregnant mare's serum gonadotropin (PMSG) and follicle stimulating hormone (FSH) in the non-breeding season of river buffaloes of the Miankaleh Peninsula, northern Iran |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 3 |
| Page no. | 189-195 |
| Keyword | River buffaloes,Bubalus bubalis,Serum,Gonadotropin,PMSG,Follicle stimulating hormone,FSH,Superovulation,Miankaleh Peninsula,Iran |
| ISSN | 1256726 |
| Abstract | Ten primiparous river buffaloes of the Miankaleh peninsula, northern Iran in the non-breeding season from the native herds were selected. The buffaloes were divided into two groups (n=5) and were injected intramuscularly with gonadotropin releasing hormone (GnRH) and a controlled internal drug-release device (CIDR) inserted on the first day. Ten days later, administration of follicle stimulating hormone (FSH) in the first group and pregnant mare's serum gonadotropin (PMSG) in the second group based on determined protocols were done. Concurrent to prostaglandin administration, CIDRs were removed and followed by estrus detection. Artificial insemination was done twice a day in both groups. Ovarian activity was assayed by ultrasonography on the first and fifth day after the start of the superovulation program. Buffaloes in which no oocyte/embryo was recovered by uterine flushing were considered non responsive to the embryo collection and were not injected with PGF2 alpha. Fifty days after insemination, pregnancy detection was performed by rectal ultrasonography. The mean (+-standard deviation, SD) of follicles diameter on right ovaries in the first day in the FSH group (9.5+-1.8 mm) was less than in the PMSG group (11.1+-1.1 mm, P=0.006). The mean (+-SD) number of follicles on both ovaries in the fifth day in the FSH group (7.4+-0.8) was signifi cantly greater than that in the PMSG group (5.0+-2.0, P=0.032). However, no oocyte/embryo was recovered in either group. Pregnancy detection 50 days after insemination showed that one cow of the FSH group and four cows of the PMSG group were pregnant. In conclusion, the PMSG and the FSH regimes induced follicular growth, but embryo recovery was poor during the non-breeding season in river buffaloes of the Miankaleh peninsula. |
