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Sequence characterization and polymorphism detection in the bubaline CD14 gene |
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| รหัสดีโอไอ | |
| Creator | 1. Aruna Pal 2. Arjava Sharma 3. Bhattaharya, T.K. 4. Mitra, A. 5. Chatterjee, P.N. |
| Title | Sequence characterization and polymorphism detection in the bubaline CD14 gene |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 2 |
| Page no. | 138-156 |
| Keyword | Buffaloes,Bubalus bubalis,CD14 gene,Exon,Polymorphism,SSCP,Sequence characterization,India |
| ISSN | 1256726 |
| Abstract | The present study was undertaken with the objectives of sequence characterization and identifi cation of polymorphisms in the bubaline CD14 gene. Single strand conformation polymorphism analysis revealed a total of eight different variants viz. CD14-A, CD14-B, CD14-C, CD14-D, CD14-E, CD14-F, CD14-G and CD14-H in exon 2 of the CD14 gene for four breeds of buffalo. Variant CD14-A was observed to be the wild type as it exhibits highest frequencies, whereas CD14-D was observed to be genetically distant from others. Polymorphism or variability may be regarded to be the highest in the Mehsana breed of buffalo, which exhibited six genotypes out of eight. Thus 42 SNPs were identifi ed for the CD14 gene of buffalo. The CD14 gene ranging from the 592nd to the 856th nucleotide of the second exon, which corresponds to the 198th to the 285th codon of the coding sequence was found to be highly polymorphic and may be regarded as the 'mutational hot spot', or the hyper variable site, leading to ligand diversity. Comparison of nucleotide sequences of different regions of the buffalo CD14 gene with that of taurine cattle revealed a total of 22 point mutations, of which eleven were non-synonymous codon with eleven amino acid substitutions. The 258 bp fragment of the CD14 gene ranging from the 3rd nucleotide to the 261st nucleotide of the 2nd exon of the CD14 gene revealed identical nucleotide sequences, indicating monomorphism. |
