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Sox-2 gene expression pattern in stem cells derived from different stages of in vitro produced buffalo (Bubalus bubalis) embryos |
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| รหัสดีโอไอ | |
| Creator | 1. Srinivasa Prasad, Ch. 2. Palanisamy, A. 3. Satheshkumar, S. 4. Gomathy, V.S. 5. Dhinakar Raj, G. 6. Thangavel, A. |
| Title | Sox-2 gene expression pattern in stem cells derived from different stages of in vitro produced buffalo (Bubalus bubalis) embryos |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2556 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 32 |
| Journal No. | 2 |
| Page no. | 131-137, 156 |
| Keyword | Buffaloes,Bubalus bubalis,Stem cells,Embryos,Sox-2,Gene expression,India |
| ISSN | 1256726 |
| Abstract | This study aims to compare the Sox-2 gene expression in stem cells derived from various stages of in vitro produced buffalo embryos. Primers were designed based on the Sox-2 sequence (NCBI Ac. No: DQ487021.1) of Chinese swamp buffalo available in Pubmed GenBank by a Web-based primer3 designing programme to obtain a product of 413bp. For zonalysis and subsequent isolation of ES cells 0.5 percent pronase was used. The DNA sequence of the RT-PCR product submitted to NCBI Pubmed GenBank was given accession number: EU661361. Strong Sox-2 expression was observed in the inner cells obtained from 16-cell stage embryos, morulae and inner cell masses of blastocyst. Out of six trials, in two trials the blastomeres/ inner cells of 2-cell, 4-cell and 8-cell stage embryos did not express Sox-2 gene even though they were believed to be totipotent. But in four trials a faint band was observed. The Sox-2 gene expression pattern was low and variable in stem cells derived from early embryos but gradually became more regular, with 100 percent expressing Sox-2 from the 16-cell stage onward. This might be related to the exhaustion of maternally generated Sox-2 transcripts and then its recovery via expression of zygotic transcripts, which takes place in buffalo embryos at the 8-16 cell stage. Epigenetic mechanisms might be the cause of the low levels of Sox-2 gene expression after fertilization. Based on the results it was believed that Sox-2 was co-expressed with Oct-4 in the ES cells and acts synergistically with Oct-4 to activate Oct-Sox enhancers, which regulate the expression of pluripotent stem cell-specifi c genes, including Nanog, Oct-4 and Sox-2 itself. |
