Isolation of exfoliated somatic cells from buffalo milk
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Creator 1. Dang, A.K.
2. Joydip Mukherjee
3. Manu Jamwal
4. Surender Singh
5. Mohanty, A.K.
6. Shiv Prasad
7. Suman Kapila
8. Rajeev Kapila
Title Isolation of exfoliated somatic cells from buffalo milk
Publisher International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand)
Publication Year 2556
Journal Title Buffalo Bulletin
Journal Vol. 32
Journal No. 1
Page no. 53-58
Keyword Buffaloes,Bubalus bubalis,Somatic cells,Milk,Neutrophils,Macrophages,Lymphocytes,Epithelial cells,India
ISSN 1256726
Abstract A technique for isolation of leucocytes (neutrophils, macrophages and lymphocytes) and epithelial cells from buffalo milk was successfully carried out. Higher fat and total solids in buffalo interfere with the isolation of milk cells, but an increase in the duration and speed of centrifugation helps in the successful isolation of these cells. Isolation of epithelial and milk leucocytes from milk is a non-invasive, easily repeatable technique which allows frequent sampling and can be used to study the immune and synthetic activity of exfoliated milk cells in vitro.
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