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การทำให้อาหารเลี้ยงเชื้อแบคทีเรียปลอดเชื้อโดยใช้เตาไมโครเวฟครัวเรือน |
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| รหัสดีโอไอ | |
| Creator | กอบชัย ภัทรกุลวณิชย์ |
| Title | การทำให้อาหารเลี้ยงเชื้อแบคทีเรียปลอดเชื้อโดยใช้เตาไมโครเวฟครัวเรือน |
| Contributor | วีระศักดิ์ จงเฟื่องปริญญา วัชรี จาดไร่ขิง |
| Publisher | King Mongkut’s University of Technology Thonburi |
| Publication Year | 2564 |
| Journal Title | KMUTT Research and Development Journal |
| Journal Vol. | 44 |
| Journal No. | 4 |
| Page no. | 577-588 |
| Keyword | Microwave, Autoclave, Sterilization, Culture Media |
| URL Website | https://journal.kmutt.ac.th/ |
| Website title | เว็บไซต์วารสารวิจัยและพัฒนา มจธ. |
| ISSN | 2697-5521 |
| Abstract | Sterilization of culture media generally involves the use of an autoclave operatingat 121oC at a pressure of 15 lb/in.2 for 20 min. Such a process takesabout 2 hours, implying that it is a time- and energy-consuming process andtherefore not suitable for preparation of a small amount of media. Microwaveoven has been reported as an equipment capable of efficiently destroyingmicroorganisms within very short time. Therefore, this research aimed to usea 1200-W household microwave for sterilization of Nutrient Broth (NB) andNutrient Agar (NA). Microwave irradiation at 1200 W for 180 seconds after themedia had boiled could well sterilize the culture media. Growth of Escherichiacoli and Staphylococcus aureus in the microwaved media was noted to bethe same as in the autoclaved media. Morphology of the cells cultured inmicrowaved media remained unchanged. Sugar utilization patterns of boththe cells cultured in microwaved as well as those in the autoclaved mediawere similar. Power consumption and time consuming for media sterilizationby microwave were less than those by autoclaving. Therefore, utilization ofmicrowave is suitable for sterilization a small volume of culture media. |
