Hydrogen Production by Unicellular Green Alga Chlorella sp. LSD-W2 Isolated from Seawater in Thailand
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Creator 1. Nichanan Tinpranee
2. Aran Incharoensakdi
3. Saranya Phunpruch
Title Hydrogen Production by Unicellular Green Alga Chlorella sp. LSD-W2 Isolated from Seawater in Thailand
Publisher Research and Technology Transfer Affairs Division
Publication Year 2559
Journal Title KKU Research Journal
Journal Vol. 21
Journal No. 28 (Supplement)
Page no. 256-266
Keyword Hydrogen production,marine green algae,heterotrophic cultivation
ISSN 0859-3957
Abstract Green algae are able to convert the unlimited sunlight energy to produce hydrogen via photosynthesis. In seawater, several kinds of marine microalgae are widespread and abundant and have been shown to tolerate and survive under the extreme salt concentrations. This work aimed to study the screening of high H2 producing marine green algal strains isolated from the Gulf of Thailand and the Andaman Sea, and the selection of the highest H2 producing strain. Its H2 production was investigated under photoheterotrophic cultivation. The result revealed that among 20 marine green algal strains, the green alga Chlorella sp. LSD-W2 gave the highest H2 production rate in both light and dark anaerobic conditions. During photoheterotrophic cultivation Chlorella sp. LSD-W2 was rapidly grown in TAP (Tris-Acetate-Phosphate) medium and reached the stationary growth phase after 36 h of cultivation. The highest photohydrogen production rate was found in cells incubated in NH 4Cl-deprived TAP medium. It was approximately 20-fold higher than H2 production rate of cells in a normal TAP medium.
Asia-Pacific Journal of Science and Technology

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