Optimization conditions, antibacterial and antioxidant activities of Clitopilus chalybescens
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Creator 1. Kritsana Jatuwong
2. Kevin D Hyde
3. Sunita Chamyuang
4. Kenji Matsui
5. Pattana Kakumyan
Title Optimization conditions, antibacterial and antioxidant activities of Clitopilus chalybescens
Publisher Research and Technology Transfer Affairs Division
Publication Year 2559
Journal Title KKU Research Journal
Journal Vol. 21
Journal No. 4 (Supplement)
Page no. 42-50
Keyword Clitopilus,optimal condition,antibacterial activity,antioxidant activity
ISSN 0859-3957
Abstract The aim of this work was to study the effect of media, temperature and pH on the mycelial growth of Clitopilus chalybescens strain MFLUCC 130809 collected from Lampang, Thailand. The antioxidant and antibacterial activities from the crude mycelial extracts were evaluated. The study indicated that the optimal medium, pH and temperature were observed on yeast extract agar (YEA) pH 5 to 7, and 2029?C, respectively. For antibacterial activities, crude extracts from mycelium slightly inhibited the growth of Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus), but no activities were observed on Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Antioxidant assay indicated that the crude extract had noticeable scavenging activity on 2,2-diphenyl-1 picrylhydrazyle (DPPH) radical. The results suggest that the crude extract from this mushroom mycelium deem to have a potential for further development on antibacterial and antioxidant applications.
Asia-Pacific Journal of Science and Technology

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