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Exobiopolymer production of Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694; optimization and scale-up |
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| รหัสดีโอไอ | |
| Creator | 1. Prathumpai W 2. Rachtawee P |
| Title | Exobiopolymer production of Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694; optimization and scale-up |
| Publisher | Research and Technology Transfer Affairs Division |
| Publication Year | 2559 |
| Journal Title | KKU Research Journal |
| Journal Vol. | 21 |
| Journal No. | 7 (Supplement) |
| Page no. | 68-80 |
| Keyword | Exobiopolymer,Phytocordyceps,Akanthomyces,general factorial design,enthomopathogenic fungi |
| ISSN | 0859-3957 |
| Abstract | Production of exobiopolymers by Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694, interleukin-8 (IL-8) inducers, were optimized using experimental design. Biological and physiological properties of these exobiopolymers are attractive to use as wound-dressing material, so that it is very interesting to increase its production. Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694 were cultivated on different carbon and nitrogen sources and the best carbon and nitrogen sources for exobiopolymer production of Phytocordyceps sp. BCC 2744 were glucose and peptone, respectively, and 0.88 g/L exobiopolymer was obtained. Higher exobiopolymer (1.82 g/L) was obtained on glucose and meat extract by Akanthomyces pistillariiformis BCC 2694. After the effects of 4 variables were studied using a two-level fractional design, glucose and peptone concentration were the most influential parameters on exobiopolymer production of Phytocordyceps sp. BCC 2744. Lower exobiopolymer production was obtained in the medium supplemented with 5-Fluorouracil and vitamin solution at high level (10 mM and 3mL/L, respectively). The highest exobiopolymer production was obtained on 60 g/L glucose and 20 g/L peptone and 2.32 g/L exobiopolymer was produced. About 4.0 g/L exobiopolymer production in a 20 L bioreactor was obtained. At least 4 variables; medium type, nitrogen sources, glucose, and nitrogen concentration were applied on a two-level factorial design of biomass and exobiopolymer production of Akanthomyces pistillariiformis BCC 2694. Models obtained from the multiple regression are significant. At high level of glucose (60 g/L), phosphate medium, and 20 g/L meat extract, higher exobiopolymer production and about 2.54 g/L was obtained. About 4.5 g/L exobiopolymer production in 20 L bioreactor was obtained. |
