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Antioxidant and Cytotoxic Activities of Mangosteen Garcinia mangostana Pericarp Extracts |
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| รหัสดีโอไอ | |
| Creator | 1. Jinnawat Manasathien 2. Piyanut Khanema |
| Title | Antioxidant and Cytotoxic Activities of Mangosteen Garcinia mangostana Pericarp Extracts |
| Publisher | Research and Technology Transfer Affairs Division |
| Publication Year | 2558 |
| Journal Title | KKU Research Journal |
| Journal Vol. | 20 |
| Journal No. | 4 |
| Page no. | 381-392 |
| Keyword | Garcinia mangostana,antioxidant activity,antiproliferation,apoptosis |
| ISSN | 2465-3986 |
| Abstract | This study was designed to evaluate the total phenolic content (TPC), antioxidation, antiproliferation and apoptotic properties of mangosteen (Garcinia mangostana L.) pericarp ethanolic and water extracts (MPE/e and MPE/w) using human breast adenocarcinoma cell line, MCF-7, as a model system. Folin-Ciocalteu method was used for TPC analysis and DPPH as well FRAP were used for antioxidant capacity. Cell viability was evaluated by Resazurin and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays for 12 and 24 h. The study found that TPC of MPE/e was over than MPE/w (119.95 ? 1.03 > 84.40 ? 0.29 ?g GAE/mg, respectively). Consistently, the antioxidant capacity of MPE/e was higher than MPE/w about 2 folds, which expressed via free radical scavenging, IC50 of 1,199.85 ? 47.16 and 2,435.71 ? 273.74 ?g/ml and FRAP, IC50 of 546.98 ? 26.12 and 1,088.70 ? 81.54 ?g/ml. Moreover, only MPE/e expressed as a potential inhibitor on the MCF-7 cells proliferation at dose- and time- dependent manner. MPE/e was strongly positive influence on antiproliferation and apoptotic induction of MCF-7 cells as seen via the morphological changes and oligonucleosomal DNA fragments. In conclusion, MPE/e may be proposed to develop to cytotoxic agents or antitumor if it has been investigated that effective and non-toxic to normal cells of animals and humans. |
