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Carotenoid composition in rotifers (Brachionus rotundiformis) enriched with three different marine microalgae species (Tetraselmis gracilis, Isochrysis galbana and Nannochloropsis oculata) |
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| รหัสดีโอไอ | |
| Creator | Amonrat Kanokrung |
| Title | Carotenoid composition in rotifers (Brachionus rotundiformis) enriched with three different marine microalgae species (Tetraselmis gracilis, Isochrysis galbana and Nannochloropsis oculata) |
| Contributor | Rawiwan Watanadilok, Tikomporn Namkorn, Bundit Plungdee |
| Publisher | Faculty of Agriculture |
| Publication Year | 2569 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 54 |
| Journal No. | 5 |
| Page no. | 1194-1208 |
| Keyword | carotenoids, rotifer, marine microalgae, HPLC and Beta-carotene |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | The cultivation of rotifers (Brachionus rotundiformis) presents a remarkable opportunity to effectively produce large quantities of vital live food. This organism is essential for young aquatic animals, providing crucial carotenoids that support growth and immune development while serving as precursors for vitamin A. Since rotifers cannot synthesize carotenoids on their own, they rely on their diet, making microalgae an indispensable source. Our research focuses on the total carotenoid content, type, and quantities of key carotenoids (lutein, astaxanthin, zeaxanthin, canthaxanthin, and beta-carotene) in rotifers supplemented with three microalgae species: Tetraselmis gracilis, Isocrysis galbana, and Nannochloropsis oculata. In this study, a completely randomized design (CRD) was applied with four experimental treatments and three replications: rotifers (B. rotundiformis) supplemented with T. gracilis at 5 × 105 cells/ml (T1), rotifers supplemented with I. galbana at 8 × 105 cells/ml (T2), rotifers supplemented with N. oculata at 10 × 105 cells/ml (T3), and a control group of unsupplemented rotifers (T4). Each experimental group used rotifers at a density of 50 individuals per milliliter and was enriched for 12 hours. The results indicate that rotifers enriched with T. gracilis exhibited a total carotenoid content of 18.46 + 4.46 µg/g wet weight, which was statistically significant (P<0.05). The identification and quantification of individual carotenoid compounds were conducted using high-performance liquid chromatography (HPLC). The results showed that astaxanthin and zeaxanthin were significantly higher in rotifers supplemented with I. galbana, with concentrations of 0.06 ± 0.02 and 0.42 ± 0.10 µg/g wet weight, respectively (P<0.05). T. gracilis also provided lutein, canthaxanthin, and beta-carotene at 1.14 + 0.21, 0.60 + 0.03, and 0.40 + 0.08 µg/g wet weight, respectively (P<0.05). The research concluded that rotifers supplemented with T. gracilis had higher total carotenoids (lutein, canthaxanthin, and beta-carotene) than other experimental treatments. This makes them particularly beneficial for aquaculture. The findings of this study present an alternative use of nutritionally enriched rotifers in aquaculture, which can enhance growth and increase the survival rates of aquatic animals during the larval stage in closed aquaculture systems and various water sources. This approach could improve feed management efficiency, offering benefits to both aquaculture farmers and commercial hatcheries. |
