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Comparative evaluation of post-thawed frozen semen quality of American Brahman and Charolais bulls using Computer-Assisted Sperm Analysis (CASA) |
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| รหัสดีโอไอ | |
| Creator | Wiwat Pattanawong |
| Title | Comparative evaluation of post-thawed frozen semen quality of American Brahman and Charolais bulls using Computer-Assisted Sperm Analysis (CASA) |
| Contributor | Yaowalak Wangsuk, Wannaluk Thaworn, Phacharaporn Tadee, Patcharee Promtan, Nittaya Thongtip, Pakasinee Khaodang |
| Publisher | Faculty of Agriculture |
| Publication Year | 2569 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 54 |
| Journal No. | 1 |
| Page no. | 54-65 |
| Keyword | frozen semen, sperm, American Brahman, Charolais |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| ISSN | 3027-6497 (Online) |
| Abstract | This study aimed to compare the post-thaw quality of frozen semen from American Brahman and Charolais bulls using Computer-Assisted Sperm Analysis (CASA). Frozen semen from 8 American Brahman and 8 Charolais bulls, with 10 straws per bull, was thawed at 37 °C for 30 seconds and evaluated for total motility, progressive motility, sperm morphology, and kinematic parameters, including linearity, beat-cross frequency, amplitude of lateral head displacement, and the ratio of straight-line velocity to curvilinear velocity. The results showed that sperm from American Brahman bulls had significantly higher progressive motility and linearity compared to Charolais bulls (P<0.05), whereas Charolais bulls exhibited significantly higher beat-cross frequency than American Brahman bulls (P<0.05). No significant differences were observed in total motility or other evaluated parameters (P>0.05). These findings indicate some breed-specific differences in post-thaw semen quality, which can be used to support the selection of suitable sires for artificial insemination and genetic improvement of beef cattle under tropical conditions in Thailand. |