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The verification and confirmation of ectomycorrhizal mushroom colonize the roots of their hosts and percentages of ectomycorrhizal after inoculating and planting in natural conditions |
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| รหัสดีโอไอ | |
| Creator | Tanapak Inyod |
| Title | The verification and confirmation of ectomycorrhizal mushroom colonize the roots of their hosts and percentages of ectomycorrhizal after inoculating and planting in natural conditions |
| Contributor | Thanakorn Lattirasuvan, Khanittha Chawananorasest, Thanapat Termarom, Chatree Konee, Natthapach Srihanant, Piyada Eamprasong |
| Publisher | Faculty of Agriculture |
| Publication Year | 2569 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 54 |
| Journal No. | 2 |
| Page no. | 430-445 |
| Keyword | Phlebopus portentosus, Astraeus odoratus, Amanita hemibapha, nucleotide sequences, ectomycorrhiza, specific primers |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | This study aimed to examine the ectomycorrhizal fungi colonization on host plant roots, and the percentage of ectomycorrhizal root formation after inoculation and planting in natural field conditions. The experiment was conducted with Phlebopus portentosus, Astraeus odoratus, and Amanita hemibapha on various host plants. After planting for a year period, the roots inoculated with P. portentosus and A. odoratus exhibited ectomycorrhizal structures with hyphae attached to the root surface, accounting for an average ectomycorrhizal formation rate between 66.24%-63.79% and 50.40%-63.86%, respectively. Those of A. hemibapha after a six-month planting period presented the percentage of ectomycorrhizal root formation on average of 31.60%-39.24%, it was considered significantly higher amount than the control plants. In addition, the molecular biological techniques using specific primers revealed the DNA bands that confirmed the presence of all three mushroom species in the host plant roots. This experiment demonstrates that the use of biomolecular methods can accurately confirm the type of mycorrhizal fungus, and it is suitable for identifying specimens that cannot be classified using morphological characteristics. |
