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In vitro callus induction of lotus embryo |
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| รหัสดีโอไอ | |
| Creator | Supanath Kanjanawattanawong |
| Title | In vitro callus induction of lotus embryo |
| Contributor | Wannakorn Sriton, Raheema Wamaedeesa |
| Publisher | Faculty of Agriculture |
| Publication Year | 2567 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 52 |
| Journal No. | 5 |
| Page no. | 911-923 |
| Keyword | embryo culture, tissue culture, Nelumbo nucifera, secondary metabolite, plant stem cell |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | The lotus (Nelumbo nucifera) is a tropical flowering plant widely utilized as a beautiful flower as well as a therapeutic herb. The lotus embryo, known as "Dee Bua" in Thai traditional medicine, has many health advantages, including lowering blood pressure and enlarging blood vessels. However, because of seasonal effects and environmental factors, led the plant became dormancy; brought to the unique flowering season, lotus embryo supply and continuity are restricted. As a result, this research project aims to investigate the production of callus development from aseptically grown lotus embryos as a potential future strategy for manufacturing secondary metabolites. The findings revealed that different surface sterilization procedures including dipping in 95% ethyl alcohol and burning with frame or soaking in Haiterยฎ with different concentrations and times produced explants with no contamination and 100% survival explant. Using Murashige and Skoog (MS) media supplemented with various PGRs, the effects of plant growth regulators (PGRs) on callus induction were investigated. A callus induction rate of 66.67% was achieved using a combination of 2,4-D (2.20 mg/L) and BAP (0.11 mg/L), with an average callus size of 0.48 cm. Furthermore, as compared to other examined techniques, the generated callus showed substantial increases in shoot length (1.12 cm) and leaf petiole length (0.99 cm). Following that, the effect of different LED lights (blue, red, violet, and dark) on callus growth and development was explored, and it was discovered that diverse light sources had no significant effect on callus induction and development (p<0.05). The callus survival rate ranged from 72.50% to 90.00%, whereas the callus formation rate ranged from 27.50% to 49.16%, and callus weight/explant ranged from 0.04-0.07 g. |
