|
Development of demethylwedelolactone and wedelolactone (in Eclipta prostrata) analysis by HPLC PDA and method validation |
|---|---|
| รหัสดีโอไอ | |
| Creator | Pradup Mesawat |
| Title | Development of demethylwedelolactone and wedelolactone (in Eclipta prostrata) analysis by HPLC PDA and method validation |
| Publisher | Faculty of Science, Ubon Ratchathani University |
| Publication Year | 2565 |
| Journal Title | Journal of Science and Science Education |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 85-96 |
| Keyword | Demethylwedelolactone, Wedelolactone, High performance liquid chromatography PDA |
| URL Website | https://so04.tci-thaijo.org/index.php/JSSE |
| Website title | https://so04.tci-thaijo.org/index.php/JSSE/article/view/256316/175223 |
| ISSN | ISSN 2697-410X |
| Abstract | This research aimed to develop and validate the method for the analysis of demethylwedelolactone and wedelolactone in extracted Kameng samples by HPLC PDA. Various types of Kameng extractions were examined. The optimum condition for separation of demethylwedelolactone and wedelolactone was 99.9% (v/v) methanol and 0.05% (v/v) formic acid at the ratio 95:5 (v/v) as mobile phase. The mode of operation was gradient elution. The results of method validation to analyze demethylwedelolactone and wedelolactone were found as follows: the limit of detection (LOD) 0.41 ?g L-1 and 0.004 ?g L-1, respectively, and the limit of quantification (LOQ) 1.37 ?g L-1 and 0.013 ?g L-1 respectively. The linearity of demethylwedelolactone in the working range was 5.0 25 ?g L-1 (r2=0.998), and wedelolactone in the working range was 1.0 25 ?g L-1 (r2=0.999). The method validation of precision for intraday precision of demethylwedelolactone and wedelolactone were found as 0.38 1.61 and 1.18 1.46, respectively. The intraday precision of demethylwedelolactone and wedelolactone were found as 0.34 0.47 and 0.59 1.20 respectively. The percentage of relative standard deviation (%RSD) and accuracy was performed by the spiked standard method. The recovery of demethylwedelolactone and wedelolactone were found as 89.21 97.00% and 100.52 100.81% respectively. For standard addition method, the recovery of demethylwedelolactone and wedelolactone were found as 103.01 104.93% and 89.69 105.94% respectively. The recovery of demethylwedelolactone and wedelolactone from both methods was acceptable. Optimum extraction condition of demethylwedelolactone and wedelolactone from Kameng were investigated using maceration, ultrasound-assisted extraction and decoction. The results show that contents of demethylwedelolactone obtained from the three extraction methods were not significantly different, whereas wedelolactone was found only in maceration and ultrasound-assisted methods. |
