Comparative leaf epidermal anatomy of some species of the family Rubiaceae in Roi Et Rajabhat University
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Creator Sutarat Khonkayan
Title Comparative leaf epidermal anatomy of some species of the family Rubiaceae in Roi Et Rajabhat University
Contributor Benyapa Ponsri, Panida Taochalee
Publisher Mahasarakham University
Publication Year 2569
Journal Title Journal of Science and Technology Mahasarakham University
Journal Vol. 45
Journal No. 1
Page no. 26-36
Keyword Plant anatomy, leaf epidermis, Rubiaceae
URL Website https://li01.tci-thaijo.org/index.php/scimsujournal
Website title Journal of Science and Technology Mahasarakham University
ISSN 1686-9664 (Print), 2586-9795(Online)
Abstract Anatomical characteristics of plants are important data for taxonomic studies. Additionally, there is little basic dataavailable to be used for identifying species in the Rubiaceae family. The objective of this research is to identify thecharacteristics of leaf epidermal tissue that can be used as a taxonomic tool for species identification. Leaf epidermalcharacteristics were examined in 20 species across 14 genera of the Rubiaceae family found at Roi Et RajabhatUniversity. The species investigated included two species each of and Spermacoce, each of Gardenia, Hedyotis,Ixora, Morinda, Oldenlandia and Spermacoce and one species each of Catunaregam, Cynanchum, Hamelia, Mitragyna, Mitracarpus, Mussaenda, Paederia and Rothmannia. Specimens were prepared using leaf epidermal peeling and clearing techniques and stained with 1% safranin. The generalized anatomical characteristics of the family were as follows: 1) Epidermal cell shapes were polygonal, irregular and jigsaw- like; 2) Stomata were of the paracytic type; 3) Trichome types included multicellular hairs, unicellular hairs and peltate hairs; 4) The cuticle patterns were either smooth, papillose or striate; and 5) Inclusion types included druse crystals, prismatic crystals, raphide crystals, sand crystals, styloid crystals, oil bodies and red staining inclusions cell. These leaf features have significant taxonomic value and can be effectively used to identify specific species or genera within the Rubiaceae family.
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