First cytogenetic report of Malayan dark-side frog (Sylvirana malayana)
รหัสดีโอไอ
Creator Thanisorn Srivorradathpisan
Title First cytogenetic report of Malayan dark-side frog (Sylvirana malayana)
Contributor Sitthisak Jantarat, Weera Thongnetr, Somsak Buatip, Thaintip Kraiprom
Publisher Mahasarakham University
Publication Year 2569
Journal Title Journal of Science and Technology Mahasarakham University
Journal Vol. 45
Journal No. 2
Page no. 196-202
Keyword Cytogenetics, karyotype, Sylvirana malayana, Hala-Bala forest
URL Website https://li01.tci-thaijo.org/index.php/scimsujournal
Website title Journal of Science and Technology Mahasarakham University
ISSN 1686-9664 (Print), 2586-9795(Online)
Abstract The first report of karyotype and idiogram of Malayan dark-side frog (Sylvirana malayana), samples collected from Hala-Bala forest, Yala Province. Chromosomes were prepared directly from bone marrow. Use the direct method with colchicine solution. Conventional chromosome staining with 20% Giemsa was dyed to study the number, type, and size of chromosomes. The results of the study revealed that the Sylvirana malayana has a diploid chromosome number (2n) = 26, with a basic chromosome number (NF) = 52, consisting of 2 large metacentric chromosomes and a submetacentric chromosome. There were 6 large, 2 medium metacentric,10 small metacentric, 4 small submetacentric and 2 small acrocentric chromosomes. No sex chromosomes could be identified. The karyotype formula for a Sylvirana malayana can be written as follows: 2n (26) = Lm2+Lsm6+Mm2+Sm10+Ssm4+Sa2 .
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