|
Effects of BA and IAA on callus induction from leaves and embryogenesis of Tacca leontopetaloides (L.) Kuntze In Vitro conditio |
|---|---|
| รหัสดีโอไอ | |
| Creator | Premjit Rongsawat |
| Title | Effects of BA and IAA on callus induction from leaves and embryogenesis of Tacca leontopetaloides (L.) Kuntze In Vitro conditio |
| Contributor | Thitikorn Prombanchong, Worawitoo Meesook, Sukjai Prommas, Komsun Nuntasoontorn |
| Publisher | Mahasarakham University |
| Publication Year | 2569 |
| Journal Title | Journal of Science and Technology Mahasarakham University |
| Journal Vol. | 45 |
| Journal No. | 2 |
| Page no. | 188-195 |
| Keyword | Tacca leontopetaloides (L.) Kuntze, callus, benzyladenine (BA), indole-3-acetic acid (IAA), embryogenesis |
| URL Website | https://li01.tci-thaijo.org/index.php/scimsujournal |
| Website title | Journal of Science and Technology Mahasarakham University |
| ISSN | 1686-9664 (Print), 2586-9795(Online) |
| Abstract | Tacca leontopetaloides (L.) Kuntze, a tuberous plant species, holds significant potential in nutrition, medicine, and industrial applications. Its importance in the food and pharmaceutical industries necessitates the development of effective propagation methods, particularly tissue culture under sterile conditions, for the extraction of bioactive compounds and production of various products. This study investigated the effects of different concentrations of benzyladenine (BA) and indole-3-acetic acid (IAA) on callus induction and embryogenesis development. Leaf segmentswere cultured on MS medium supplemented with BA at concentrations of 0, 0.5, 1.0, and 1.5 mg/L, combined with IAA at concentrations of 0, 0.5, 1.0, and 1.5 mg/L. The experiment followed a completely randomized design (CRD) with three replications, and the differences in means were analyzed at a 95% confidence level. After four weeks of culture, callus formation was observed in the leaf segments, with the callus exhibiting a compact green appearance.The highest callus induction rate (100%) was achieved on MS medium containing 1.0 mg/L BA and 1.0 mg/L IAA, which was significantly different from other treatments. For callus development into plantlets, MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L IAA produced the highest number of shoots (5.67 ± 0.72 shoots) and the greatestaverage number of roots (3.07 ± 0.80 roots) after four weeks of culture. The results demonstrate that the combination of BA and IAA at optimal concentrations is effective in promoting callus induction and subsequent shoot and rootdevelopment in Tacca leontopetaloides (L.) Kuntze. |
