|
The extraction of estrogen-like compounds from Pueraria mirifica, the properties characterization and the preparation of nucleated nanoparticles |
|---|---|
| รหัสดีโอไอ | |
| Creator | Wachira Yodthong |
| Title | The extraction of estrogen-like compounds from Pueraria mirifica, the properties characterization and the preparation of nucleated nanoparticles |
| Contributor | Saransiri nuanmanee, Phenphichar Wanachantararak, Nattakanwadee Khumpirapang, Tanongsak Sassa-deepaeng |
| Publisher | Faculty of Science and Agricultural Technology |
| Publication Year | 2563 |
| Journal Title | Journal of Science and Agricultural Technology |
| Journal Vol. | 1 |
| Journal No. | 2 |
| Page no. | 7-13 |
| Keyword | Pueraria mirifica, antioxidant, MCF-7, polymeric micelles, nanoparticles |
| URL Website | https://ph02.tci-thaijo.org/index.php/JSAT/index |
| Website title | JSAT |
| ISSN | 2730-1524 |
| Abstract | Pueraria mirifica (PM), also known as Kwao Krua Khaw, has been used as an herbal plant for manydecades in the Northern part of Thailand. The root of PM contains estrogen-like compounds that are less soluble inwater resulted in low bioavailability in an aquatic animal application. To increase solubility of the compounds, sixdifferent polarity solvents such as hexane, benzene ethyl acetate, acetone, ethanol, and deionized distilled waterwere employed for extraction. It found that deionized distilled water exhibited the highest extraction percentageyield. However, a high amount of phenolic compounds and flavonoids were obtained in ethyl acetate fraction (EAF).In addition, the results of scavenging radical assay using DPPH IC50 showed the highest activity of EAF due to thehigh content of total phenolic content and flavonoids content. Moreover, EAF, significantly exhibited the highestproliferation activity against MCF-7. Therefore, EAF was selected for developing polymeric micelles. The HP?CD,Pluronic F-68, and Pluronic F-127 were employed with Tween80 to prepare suitable nanoparticles. The proper ratioof EAF-loaded Pluronic F-127 (EAF-F127) product in nanosize range elicited zeta potential value of nearly zeromV and exhibited the transparent aqueous dispersions in DI water. The results promised further in vivo studies. |
