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THE INFLUENCE OF CULTURE MEDIA ON GROWTH AND MORPHOLOGY OF SCEDOSPORIUM BOYDII AND SCEDOSPORIUM PROLIFICANS |
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| รหัสดีโอไอ | |
| Creator | Watcharamat Muangkaew, Thanwa Wongsuk, Potjaman Pumeesat, San Suwanmanee, Natthanej Luplertlop |
| Title | THE INFLUENCE OF CULTURE MEDIA ON GROWTH AND MORPHOLOGY OF SCEDOSPORIUM BOYDII AND SCEDOSPORIUM PROLIFICANS |
| Publisher | Faculty of Medicine,Srinakharinwirot University |
| Publication Year | 2559 |
| Journal Title | Journal of Medicine and Health Sciences |
| Journal Vol. | 23 |
| Journal No. | 2 |
| Page no. | 17-25 |
| Keyword | Scedosporium boydii, Scedosporium prolificans, selective media, basic culture media, morphology |
| ISSN | 0859-3299 |
| Abstract | The culture media for fungal cultivation contained several specific and various nutrients in order to support the growth of fungal. This study aimed to determine growth and morphology of Scedosporium boydii and Scedosporium prolificans on different types of culture media. The selective media and standard nutrient added media were used to study the growth rate and morphology of S. boydii and S. prolificans by adding each medium into 6 well plates. Each type of medium was used to culture both S. boydii and S. prolificans and incubated at 37 ำC condition for 4 days. The growth rate was determined by colonies diameter measurement. Morphological analysis was determined in S. boydii and S. prolificans in different types of culture medium. The results showed that colonies of S. boydii and S. prolificans on SDA+10% Fetal Bovine Serum, (FBS) exhibited larger size than colonies growing on other culture media on the fourth day of cultivation (31.67 ? 1.53 and 25.33? 2.36 mm respectively). The growth rate of S. boydii and S. prolificans was 7.92 and 6.33 mm/day respectively. The morphological study of S. boydii colony showed wide expanded, white color, and gradually changed to gray color. S. prolificans colony showed wide expanded, gray-white color, and gradually changed to gray and black color. In addition, the lactophenol cotton blue stain showed no difference in conidia shape of both S. boydii and S. prolificans (globose-subglobose shape). Therefore, this study demonstrated that SDA+10% FBS represented proper and alternative medium choice of S. boydii and S. prolificans culture for rapid growth culture system |
