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Genetic diversity of filaria in cat reservoirs |
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| รหัสดีโอไอ | |
| Creator | Arda Pakpitcharoen, Sunsanee Phasook, Paisarn Khawsak,Sintawee Khuchareontaworn and Kosum Chansiri |
| Title | Genetic diversity of filaria in cat reservoirs |
| Publisher | Faculty of Medicine,Srinakharinwirot University |
| Publication Year | 2548 |
| Journal Title | Journal of Medicine and Health Sciences |
| Journal Vol. | 13 |
| Journal No. | 1 |
| Page no. | 146-154 |
| Keyword | B. malayi, genetic diversity, reservoir host |
| ISSN | 0859-3309 |
| Abstract | Lymphatic filariasis is a significant health problem in many developing countries. InThailand, only two of parasitic worms, Wucherelia bancrofti and Brugia malayi are reported.The survey of feline blood samples in the endemic area of Narathiwat, the province in thesouth of Thailand, revealed that two of them were infected with Brugia spp. Upon theexamination of feline blood specimens by Giemsa staining, the results were positive formicrofilaria. However, morphological characters did not clearly provide a mean ofdistinguishing Brugia species. In this study, the internal transcribed spacer 1 (ITS1) andinternal transcribed spacer 2 regions (ITS2) have been isolated by using PCR amplification.generating the products of 586 bp and 660 bp in sizes, respectively. DNA sequencing,nucleotide sequence alignment and phylogenetic tree construction revealed that ITS1 andITS2 trees showed the genetic diversity of B. malayi in Cat 1 and Cat 2. In addition, the ITS1tree also indicated that the Cat 2 contained the new variant of B. malayi whereas ITS2 treedemonstrated that the mix infection of B.malayi, B. pahangi and D. repens may be occurred |
