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Diagnosis of aspergillosis by double immunodiffusion method with home-made Aspergillus antigens and antisera |
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| รหัสดีโอไอ | |
| Creator | Nittaya Tripinyipap,Anakana Chaiprasert,Suchai Charoenratanakul |
| Title | Diagnosis of aspergillosis by double immunodiffusion method with home-made Aspergillus antigens and antisera |
| Publisher | Faculty of Medicine,Srinakharinwirot University |
| Publication Year | 2540 |
| Journal Title | Journal of Medicine and Health Sciences |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Page no. | 7-11 |
| Keyword | aspergillosis, double immunodiffusion method |
| ISSN | 0859-3299 |
| Abstract | aspergillosis frequently affects respiratory tract. The definitive diagnosis is difficult. In this study, double immunodiffusion method was developed for detection of precipitating antibodies to Aspergillus species in patients sera by a home-made battery of reagent, composed of culture filtrate antigens and their homologous rabbit antisera of A.fumigatusB-1172,A. flavusB-15,A.niger 107,A.nidulansB-1390 and A. terreusB-985. Three hundred and fourty nine sera which comprised of 129 from patients with suspected aspergillosis,30 from patients with lung cancer, 18 from patients with othr systemic mycotic infactions, 34 from patients with meliodosis, 17 from patients with active pulmonary tuberculosis and 121 from normal individuals were tested. Positive precipitating antibodies by double immunodiffusion method were found in 27.13% (35/129) of patients with suspected aspergillosis while false positive results were found in 1.18%(4/220) in control groups. |
