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Diagnostic Application of Monoclonal Antibodies Against Protein Antigen of Salmonella Typhi |
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| รหัสดีโอไอ | |
| Creator | Pattarna Ekpo,Supinya Pongsunk |
| Title | Diagnostic Application of Monoclonal Antibodies Against Protein Antigen of Salmonella Typhi |
| Publisher | Faculty of Medicine,Srinakharinwirot University |
| Publication Year | 2538 |
| Journal Title | Journal of Medicine and Health Sciences |
| Journal Vol. | 2 |
| Journal No. | 2 |
| Page no. | 69-73 |
| Keyword | Salmonella Typhi |
| ISSN | 0859-3299 |
| Abstract | Ten hybrid clones producing monoclonal antibodies (MAbs) specific forSalmonella typhr were produced by using the affinity purified Salmonella typhiprotein (~p) as immunogen. The MAbs reacted specifically with the 52 kDaflagellin protein component. The MAbs were used to establish a double antibodysandwich ELISA for detection of the 52 kDa flagellin antigen in serum samplesfrom patients with acute typhoid fever. With this assay system, 6.25 ng per ml offlagellin antigen of S. typhi could be detected. However, the assay system couldnot detect the flagellin antigen in patrents sera. |
