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Mycobacterium tuberculosis detection and drug susceptibility testing using AnyplexTM Real-time PCR in comparison with standard culture |
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| รหัสดีโอไอ | |
| Creator | 1. Somsak Sintu-urai 2. Kannaphat Kesornsukhon 3. Patthama Klomporn 4. Thanakorn Phowong 5. Ratchaneeporn Khummin 6. Suwannee Keeratiwasee |
| Title | Mycobacterium tuberculosis detection and drug susceptibility testing using AnyplexTM Real-time PCR in comparison with standard culture |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2559 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 49 |
| Journal No. | 2 |
| Page no. | 218-226 |
| Keyword | Multi drug resistance TB, tuberculosis, real-time PCR, MGIT 960 |
| ISSN | 25396056 |
| Abstract | Introduction: Bacterial cultivation is the gold standard method for diagnosis of tuberculosis (TB). However, technique is time consuming for 28-56 days of cultivation. Therefore, several rapid tests have been developed. Real-time PCR is one of the quick TB test currently used for identifying both drug-susceptible and multidrug resistant TB (MDR-TB). However, the efficiency of real-time PCR compared to conventional method has not been elucidated.Objective: To compare the efficiency between real-time PCR and conventional methods including cultivation in solid media and mycobacteria Growth Indicator Tube (MGIT 960) system for detection of TB and MDR-TB.Materials and Methods: One hundred and forty-seven sputum specimens collected from hospital sectors of the Office of Disease Prevention and Control 2 in Phitsanulok were decontaminated and subjected to culture in LJ medium agar slant and MGIT 960 system. Bacterial DNA was extracted and real-time PCR was performed using the AnyplexTM MTB/NTM detection kit and AnyplexTM II MDR-TB detection kit.Results: Samples (86.2%) were positive by real-time PCR and was 39.3% higher than conventional culture. Sensitivity, specificity, positive predictive value, and negative predictive value of real-time PCR for detection of multidrug-resistant tuberculosis were 96.82%, 28.05%, 49.17%, and 92.0%, respectively, as compared to MGIT 960 system. Time required for detection of TB between MGIT 960 system and real-time PCR is 13 and 2 days. However, total cost of MGIT 960 system is cheaper than that of real-time PCR (600 Baht vs 1,500 Baht, respectively).Conclusion: Real-time PCR is the useful diagnostic test for rapid detection of MTB and MDR-TB. Real-time PCR provides higher sensitivity and specificity than convention culture methods. This method can be used as a screening test for TB infection where rapid treatment can be achieved. However, this method has to be further on evaluated for its cost effectiveness. |
