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An Outbreak of type B botulism in Chaiyaphum Province, Thailand 2014 |
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| รหัสดีโอไอ | |
| Creator | 1. Piyada Wangroongsarb 2. Chutima Jittaprasartsin 3. Karun Suthivarakom 4. Thanitchai Kamthalang 5. Nattapong Cheunban 6. Somchai Sangkitporn |
| Title | An Outbreak of type B botulism in Chaiyaphum Province, Thailand 2014 |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2558 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 48 |
| Journal No. | 1 |
| Page no. | 49-58 |
| Keyword | C. botulinum, foodborne, molecular technique |
| ISSN | 1255347 |
| Abstract | Introduction: Clostridium. botulinum strains that produce types A and B to in generally associated with severaloutbreaks in the United States, China, South Amerca and southern European countries, and the mostfrequently implicated foods are vegetables. Diagnosis of botulism has been made by detecting the neurotoxinand C. botulinum cells in patients and/or suspected food samples. The aim of this study was to identifythe cause of foodborne outbreak and analysis the symptoms of botulism in Chaiyaphum Province, 2014.Materials and methods: The samples from Chaiyaphum Province outbreak were comprised of suspected food andclinical samples from 4 patients. Total 11 samples were identified by the cultivation, mouse bioassay and typing toxingenes amplication by multiplex PCR. The subtypes B1-B8 were compared based on the amino acid sequencesalignment of bont/B1 to B8 subtypes using MEGA software which produce an unweighed pair group method witharithmetic mean (UPGMA).Results: The clinical symtoms were observed especially with blurred vision, glossoplegia, dysarthria, nausea,dyspnea and required mechanical ventilation for support breathing. The etiological agent of this foodborne botulismoutbreak detected by culturing, mouse bioassay and multiplex PCR method in fermented bamboo shoots contaminatedwas C. botulinum type B8. The phylogenetic tree of Chaiyaphum 2014 strain was constructed and the result demonstratedan identical to B8 subtype of Surat Thani 2012 strain (KC 714045).Conclusions: From this study showed that the etiologic agent of an outbreak in Chaiyaphum Province wasfermented bamboo shoot contaminated with C. botulinum type B8. Botulism disease must be considered clinicalsymptoms which was important to provide treatment of patients in time. It should combine with laboratory diagnosisin order to obtain the accurate results. |
