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Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
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| รหัสดีโอไอ | |
| Creator | 1. Aoranit Somno 2. Songyot Anuchpreeda 3. Sawitree Chiampanichayakul |
| Title | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2558 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 48 |
| Journal No. | 3 |
| Page no. | 173-181 |
| Keyword | Multidrug resistance, P-glycoprotein, survivin, CD147, K562 cell line |
| ISSN | 1255347 |
| Abstract | Introduction: Leukemia is a hematologic malignant disease. To date, chemotherapy is the most effective method for leukemia treatment. However, multidrug resistance (MDR) is a major obstacle of leukemia treatment failure. Previous studies have shown that multidrug resistance phenotype is associated with P-glycoprotein (P-gp) and survivin which is a drug transporter protein and inhibitors of apoptosis proteins (IAPs), respectively. Moreover, overexpressed CD147 has been reported to be involved in the multidrug resistance cell lines. However, the relationship of P-gp, survivin, and CD147 in leukemia with MDR phenotype have not been reported.Objective: The purpose of this study was to investigate the expressions of P-gp, survivin and CD147 in leukemic cells with MDR phenotype K562/Adr compared to drug sensitive K562 cells.Materials and methods: The sensitivity of the adriamycin on K562/Adr (drug resistance) and K562 (drug sensitive) cells were determined by MTT assay. Expressions of P-gp, survivin, and CD147 were then investigated by RT-PCR and Western blotting.Results: Adriamycin showed the cytotoxic effect on K562 cells more than K562/Adr cells with the inhibitory concentration at 50% growth (IC50) values of 2.17?1.02 ?M and >10 ?M, respectively. P-gp, survivin, and CD147 showed the significant increase in both protein and mRNA levels in K562/Adr cells by Western blot and RT-PCR.Conclusion: This study is the first report describing the high level expression of P-gp, survivin, and CD147 in leukemia with multidrug resistance phenotype. |
