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Detection of FY*A, FY*B and FY*X genotypes in Duffy blood group system by polymerase chain reaction-sequence specific primer (PCR-SSP |
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| รหัสดีโอไอ | |
| Creator | 1. Poonsub Palacajornsuk 2. Laksamee Jamrassri 3. Phiyada Kongtes |
| Title | Detection of FY*A, FY*B and FY*X genotypes in Duffy blood group system by polymerase chain reaction-sequence specific primer (PCR-SSP |
| Publisher | Faculty of Associated Medical Sciences, Chiang Mai University |
| Publication Year | 2558 |
| Journal Title | Journal of Associated Medical Sciences |
| Journal Vol. | 48 |
| Journal No. | 2 |
| Page no. | 136-143 |
| Keyword | Genotype, FY*A, FY*B, FY*X, Duffy, PCR-SSP |
| ISSN | 1255347 |
| Abstract | Research problems: Serology method is the most commonly used for Duffy blood group phenotyping. This method is simple, convenient and inexpensive. However, mutation of FYx gene at position C265T can cause a weak expression of Fyb antigen and may not be detected by anti-Fyb which lead to false negative by serology testing. The objective of this study is to detect FY*A, FY*B and FY*X genotypes in Duffy blood group system by polymerase chain reaction-sequence specific primer (PCR-SSP).Materials and methods: Ninety seven samples were tused for phenotyping of Fya and Fyb by serology method and the DNA was extracted for detect FY*A and FY*B genotype by PCR-SSP.Results: Phenotyping of 97 samples showed that 78 samples (80.41%) were Fy(a+b-) and 19 samples (19.59%) were Fy(a+b). Seventy-eight DNA samples of Fy(a+b-) were then detected for FY*X genotype at position C265T by PCR-SSP. The results showed no C265T mutation in all tested samples. The comparison results between serology and PCR-SSP methods were concordant.Conclusions: PCR-SSP can be as alternative method to detect FY*A, FY*B and FY*X genotype in Duffy blood group system for selecting suitable blood to the patient who has anti-Fya and/or anti-Fyb in order to prevent adverse effects of transfusion reaction and investigate for the cause of hemolytic disease of the newborn. |
