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Detection and Discrimination Modeling of Cells in Droplet Using Impedance Spectroscopy-Based Microfluidic System |
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| รหัสดีโอไอ | |
| Creator | Shabbir Chowdhury, Mohammad Asif Hussain, Nebras Sobahi |
| Title | Detection and Discrimination Modeling of Cells in Droplet Using Impedance Spectroscopy-Based Microfluidic System |
| Contributor | - |
| Publisher | TuEngr Group |
| Publication Year | 2565 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 13 |
| Journal No. | 3 |
| Page no. | 13A3Q: 1-17 |
| Keyword | Microfluidics, Impedance-spectroscopy, Droplet, Cell counting, Cell in the droplet, Cell detection. |
| URL Website | http://TuEngr.com/Vol13-2.html |
| Website title | ITJEMAST V13(3) 2022 @ TuEngr.com |
| ISSN | 2228-9860 |
| Abstract | Cells in the droplet can be detected and characterized using an impedance spectroscopy-based microfluidic characterization technique that is relatively low-cost and efficient and brings several benefits to biomedical and biological research. In this modeling analysis, the two types of cells were differentiated and counted using a microfluidic impedance spectroscopy-based system. For this purpose, the optimal frequency and condition were characterized and determined. The impedance difference between wild-type and mutant yeast cells was found as 20.91? for the spherical-shaped droplet. For larger microdroplets, the squeezed shape droplets were considered, and in which condition, the difference became 14.42? which makes this model practically implementable for a large number of cells in droplet studies. This model can easily count the cells in the droplets based on the electrical impedance. This cost-effective design can dramatically improve cell sorting, counting, and separation. Further, the sensitivity can be amplified after the detection period to a reasonable state if needed. |
