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Biotechnology of Cultivation of Probiotic Lactobacilli |
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| รหัสดีโอไอ | |
| Creator | Yury Lysenko, Nadezhda Machneva, Anatoly Smirnov, Alexander Panin, Andrey Koshchaev |
| Title | Biotechnology of Cultivation of Probiotic Lactobacilli |
| Contributor | - |
| Publisher | TuEngr Group |
| Publication Year | 2564 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 12 |
| Journal No. | 4 |
| Page no. | 12A4I: 1-9 |
| Keyword | Lactobacilli cultivation, Nutrient medium, Growth requirements, Reducing sugars, Microorganism's growth. |
| URL Website | http://TuEngr.com/Vol12_4.html |
| Website title | ITJEMAST V12(4) 2021 @ TuEngr.com |
| ISSN | 2228-9860 |
| Abstract | This article presents the research results on the selection of optimal conditions for the cultivation of probiotic microorganisms isolated from honey bee drone milk for increasing the biomass of the studied lactobacilli. To determine microorganisms' growth requirements, we used the developed molasses medium and conventional nutrient media for lactobacilli. As a result of calculating the number of lactic acid bacteria on agar nutrient medium, it was found that molasses medium has optimal growth requirements, which includes - 45.0 g/l of fodder molasses (50% beet molasses and 50% corn molasses), K2HPO4 - 2.0 g/l, yeast extract - 0.02 g/l. Simultaneously, the developed medium promoted the increase in the titer of microorganisms up to 5.0 x 1010 CFU/ml. The amount of the studied Lactobacillus kunkeei reached 2.34.7 x 109 CFU/ml on standard nutrient media. When studying the effect of different temperatures and growing times on lactic acid bacteria's biomass growth, the optimal cultivation conditions are 37?C and 24 h. It was proved that the maximum depletion of the nutrient medium's carbon component occurs 24-28 hr from the start of the microorganism growth process. |
