Diagnoses of Leptospirosis in Animals
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Creator Gulnara Nurlygayanova, Vasily Belousov, Alisa Varentsova, Nadezhda Ozheredova, Oleg Chernykh
Title Diagnoses of Leptospirosis in Animals
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Publisher TuEngr Group
Publication Year 2563
Journal Title International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
Journal Vol. 11
Journal No. 15
Page no. 11A15P: 1-9
Keyword Dark-Field Microscopy, Microagglutination Reaction, PCR, Bacteriological studies, Microagglutination Reaction, Infectious jaundice, Microscopic agglutination test (MAT)
URL Website http://TuEngr.com/Vol11_15.html
Website title ITJEMAST V11A(15) 2020 @ TuEngr.com
ISSN 2228-9860
Abstract There have been presenting a comparative analysis of the results of leptospirosis diagnosis by methods used in the Russian Federation's veterinary laboratories. Using the microagglutination reaction, specific antibodies to leptospira were detecting in 136862 animals. A total of 64686 urine samples was examined with dark-field microscopy. Applying PCR, the leptospira genome is detected in pathological material in 57 cases. The PCR method in the water of open reservoirs cannot detect the pathogenic leptospira genome. In the study of biological material, the leptospira genome was found in 248 cases. To increase the efficiency of diagnosis and reduce the time of diagnosis for leptospirosis, it is advisable to conduct parallel bacteriological studies of the material in combination with PCR analysis. Micro-agglutination reaction and molecular genetic analysis (PCR) are the most inforeaction micro-agglutinative and technologically advanced laboratory diagnostic methods.
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