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Effect of Gout Drug Treatments on Survival Rate and Morphological Change of Lindernia sp. in vitro |
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| รหัสดีโอไอ | |
| Creator | 1. Kamontip Sungkaew 2. Thunya Taychasinpitak 3. Shermarl Wongchaochant 4. Patana Sukprasert 5. Shinji Kikuchi |
| Title | Effect of Gout Drug Treatments on Survival Rate and Morphological Change of Lindernia sp. in vitro |
| Publisher | TUENGR GROUP |
| Publication Year | 2558 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 6 |
| Journal No. | 4 |
| Page no. | 191 |
| Keyword | Linderniaceae, Plant Mutation, Tissue culture, Colchicine treatment |
| ISSN | 2228-9860 |
| Abstract | This research created greater genetic variation in Lindernia sp. by studying the most appropriate concentration and duration of colchicine treatment that could induce polyploidy in Lindernia sp. Leaf and node sections taken from 1-month old Lindernia plants grown in vitro were used as the explants to be immersed in colchicine solution at the concentrations of 0, 5, 10, 15 and 20 ppm for 1, 2 and 3 days. The experiment was a 5?3 Factorial in CRD with 10 replications and 2 explants per replication. The results showed that the plants regenerated from the colchicine-treated node and leaf sections had lower survival rate, shorter plant height, shorter internode length, fewer roots and shorter roots than the control group, with an increasing effect from increased concentrations of colchicine and increased exposure time; but the colchicine-treated plants had thicker stems, thicker roots and wider and longer leaves than the control. The treatment group exposed to colchicine at the concentration of 10 ppm for 3 days had the lowest survival rate (60% for node sections and 50% for leaf sections), but the highest polyploidy induction rate at 22.00% for node sections and 16.22% for leaf sections. The frequency of polyploidy induction was higher in node sections than in leaf sections, and the plants regenerated from node sections were also stronger and had a higher survival rate when planted out than those regenerated from leaf sections. |
