|
Optimization of Enzymatic Clarification from Corncob |
|---|---|
| รหัสดีโอไอ | |
| Creator | SininartChongkhong |
| Title | Optimization of Enzymatic Clarification from Corncob |
| Contributor | Woraluk Kongjindamunee |
| Publisher | TUENGR Group |
| Publication Year | 2557 |
| Journal Title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 67-76 |
| Keyword | Alpha-amylase, Glucose content, Hydrolysis, Central composite design. |
| URL Website | http://tuengr.com |
| Website title | International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies |
| ISSN | 2228-9860 |
| Abstract | A major content that was 22.76% total carbohydrate of the corncob could be simply hydrolyzed into reducing sugars by using alpha-amylase. The clarification process using alpha-amylase was optimized by response surface methodology (RSM) in this work. Independent variables including: enzyme amount of 0.05-0.2 %w, time of 60-240 min and temperatures of 80-100?C were investigated. Their effects were found on the reducing sugar (Glucose content) by a second order central composite design (CCD). The optimum condition was 0.2 %w alpha-amylase, 87.6?C for 150 min. It could provide the highest amount of 6.21 g/L glucose content in the clarified product. |
