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Effects of Ya Khao, a Thai traditional herbal formula extract, on hyperglycemia and associated biochemical and histopathological changes in streptozotocin-induced diabetic rats |
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| รหัสดีโอไอ | |
| Creator | Komaraphat Pinthong |
| Title | Effects of Ya Khao, a Thai traditional herbal formula extract, on hyperglycemia and associated biochemical and histopathological changes in streptozotocin-induced diabetic rats |
| Contributor | Bungorn Sripanidkulchai, Niramai Fangkrathok, Monthaka Teerachaisaku, Wanassanun Pannangrong, Apinya Thap-iam, Pannawat Choowong-In, Nutchareeporn Nillert |
| Publisher | Phetchaburi Rajabhat University |
| Publication Year | 2569 |
| Journal Title | Interdisciplinary Research Review (IRR) |
| Journal Vol. | 21 |
| Journal No. | 1 |
| Page no. | 18-27 |
| Keyword | Ya Khao formula extract, Diabetes mellitus, Anti-hyperglycemic effect, Pancreatic protective effect, Streptozotocin |
| URL Website | https://ph02.tci-thaijo.org/index.php/jtir |
| Website title | Interdisciplinary Research Review (IRR) |
| ISSN | 2697-536X |
| Abstract | Diabetes mellitus (DM) was a chronic metabolic disorder marked by hyperglycemia and affected millions of individuals globally. Ya Khao (YK) was a Thai traditional herbal formula inscribed on the stone slabs of Wat Pho. It consisted of 15 different herbs in equal proportions and had been traditionally used to treat various fevers for a long time. This study aimed to evaluate the anti-hyperglycemic, biochemical, and histopathological effects of Ya Khao formula extract (YKFE) in streptozotocin (STZ)-induced diabetic rats. Forty male Sprague-Dawley rats were classified into 5 groups (n = 8): NDM (non-diabetic), DM, DM+GB (glibenclamide), DM+YKFE250, and DM+YKFE500. Rats in the DM groups were induced to develop the disease by receiving an intraperitoneal injection of STZ (65 mg/kg BW), whereas rats in the NDM group were injected with citrate buffer solution. All rats were administered orally once daily for 2 weeks. Body weights were monitored daily, and fasting blood glucose (FBG) levels were assessed weekly. At the end of the treatment, all rats were euthanized, and their blood samples were collected for FBG and biochemical analyses. Vital organs (pancreas, liver, and kidneys) were removed and weighed, and the pancreas was fixed in 10% formalin for histopathological evaluation. Treatment with both doses of YKFE for 2 weeks significantly decreased FBG levels in diabetic rats (* P < 0.05, ** P < 0.01 compared with the DM group) and ameliorated pancreatic histopathological changes. However, there were no differences in body weight gain and relative organ weight among the groups. Besides, the treatment duration was insufficient to normalize hepatic and renal functions to levels comparable to the NDM group. Collectively, the results suggested that YKFE exhibited anti-hyperglycemic and pancreatic protective effects in STZ-induced diabetic rats and showed potential for further development as a therapeutic agent for DM. |
