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Quantitative Determination of Bergenin in Mallotus repandus (Willd.) Muell. Arg. Stem Extract by Reverse Phase-High Performance Liquid Chromatography |
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| รหัสดีโอไอ | |
| Creator | Yollada Sriset |
| Title | Quantitative Determination of Bergenin in Mallotus repandus (Willd.) Muell. Arg. Stem Extract by Reverse Phase-High Performance Liquid Chromatography |
| Contributor | Waranya Chatuphonprasert, Kanokwan Jarukamjorn |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2561 |
| Journal Title | Isan Journal of Pharmaceutical Sciences |
| Journal Vol. | 14 |
| Journal No. | 1 |
| Page no. | 67-74 |
| Keyword | Mallotus repandus, bergenin, reverse phase-high performance liquid chromatography (RP-HPLC) |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences, IJPS |
| ISSN | 19050852 |
| Abstract | Bergenin, a polyphenol compound, is a major active constituent of Mallotus repandus (Willd.) Muell. Arg. which possesses pharmacological activities including anti-oxidant, hepatoprotective, anti-microbial, anti-inflammatory, and immunomodulatory activities. Objectives: To develop and validate the reverse phase-high performance liquid chromatography (RP-HPLC) for quantification of bergenin in M. repandus stem extract. Methods: The RP-HPLC was employed with Phenomenex Luna C18 column (250 mm x 4.6 mm, 5 mm) as stationary phase. The mobile phase consisted of acetonitrile : water (10 : 90 v/v), at a flow rate of 1 mL/min. The wavelength at 272 nm was used for detection of bergenin. Results: The retention time of bergenin was 10.885 min with no peak interference. The method at the bergenin concentration range of 20-320 mg/mL was in a good linearity (R2 = 0.9999). The accuracy measured as %recovery was 93.22 ? 1.49%. The precision of within-day and between-day expressed as %relative standard deviation were of 0.57-1.48% and 0.19-1.70%, respectively. Limit of detection and limit of quantification were 4.56 and 15.20 mg/mL, respectively. By the soxhlet extraction and maceration, the aqueous extract of M. repandus stem had the highest bergenin content of 12.67 ? 0.26% and 19.38 ? 0.63% dry% weight, respectively, followed by methanolic (9.28 ? 0.49% and 8.73 ? 0.72% ) and ethanolic extracts (2.16 ? 0.10% and 1.73 ? 0.05%), respectively. Conclusion: The RP-HPLC method for determination of bergenin was achieved with good and acceptable specificity, linearity, accuracy, precision, and sensitivity. The utilization was assured by determination of bergenin content in the M. repandus stem extracts. |
