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Bioactivities of Goat Placenta hydrolysates |
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| รหัสดีโอไอ | |
| Creator | Onanong Ruksounjik |
| Title | Bioactivities of Goat Placenta hydrolysates |
| Contributor | Sompong Klaynongsruang, Chariya Hahnvajanawong, Watcharee Khunkitti |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2559 |
| Journal Title | Isan Journal of Pharmaceutical Sciences |
| Journal Vol. | 12 |
| Journal No. | 3 |
| Page no. | 61-71 |
| Keyword | Goat placenta, protein/peptide hydrolysates, papain enzyme hydrolysis, Antioxidant activity, Ferric Reducing Power, Lipid peroxidation inhibition, Anti-tyrosinase |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences, IJPS |
| ISSN | 19050852 |
| Abstract | Introduction: Goat placenta is a rich source of proteins and amino acids. It has been reported that low molecular weights of short chain proteins and peptides might possess various bioactivities. Objectives: To investigate some bioactivities of proteins/peptide hydrolysates by papain enzyme. Methods: determination the characteristics of the goat placenta hydrolysates including total protein by Bradford protein assay, molecular weight by Tricine-Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (Tricine-SDS PAGE) and investigation of antioxidant activities e.g. Free radical scavenging (DPPH), Inhibition of Linoleic Acid Oxidation and Ferric Reducing Power as well as anti-mushroom tyrosinase activity. Results: the % yield of the hydrolysates obtained from goat placenta was 18.31?0.42%. The total protein equivalent to bovine serum albumin was 0.075?0.003 mg/ml. The majority of obtained hydrolysates contained proteins and peptides at a molecular weight of approximately 30 kDa. Antioxidant activities of the hydrolysates were as follows; Free radical scavenging of the hydrolysates at a concentration of 6.25 mg/ml was significantly greater than that of vitamin C at 0.156 mg/ml and was not significantly different from vitamin E acetate at 83.5 mg/ml but less than that of Trolox at 0.156 mg/ml. Ferric Reducing Power of the hydrolysates at 1.5 mg/ml was equivalent to 15.74 mM Trolox which was less than 0.3 mg/ml of vitamin C. Lipid peroxidation inhibition of the hydrolysates at 1 mg/ml was 84.94% which was not significantly different from that of BHT, Trolox at 0.1 mg/ml and GSH at 1 mg/ml. The hydrolysates at 41.67 mg/ml inhibited 59.11% of mushroom tyrosinase activity. Conclusion: goat placenta proteins/peptide hydrolysates possessed antioxidant activities and anti-tyrosinase activity. |
