|
Immunomodulating, Antioxidant and Antimicrobial Activities of Aqueous Extract of Bauhinia penicillilobaPierre ex Gagnep. |
|---|---|
| รหัสดีโอไอ | |
| Creator | Chutinun Prasitpuriprecha |
| Title | Immunomodulating, Antioxidant and Antimicrobial Activities of Aqueous Extract of Bauhinia penicillilobaPierre ex Gagnep. |
| Contributor | Bung-orn Sripanidkulchai, Viraphong Lulitanond |
| Publisher | Faculty of Pharmaceutical Sciences KKU MSU UBU |
| Publication Year | 2550 |
| Journal Title | Isan Journal ofPharmaceutical Sciences |
| Journal Vol. | 3 |
| Journal No. | 2 |
| Page no. | 23-34 |
| Keyword | Bauhinia penicilliloba, Immunomodulating activity, Antioxidant activity, Antimicrobial activity |
| URL Website | https://tci-thaijo.org/index.php/IJPS |
| Website title | Isan Journal ofPharmaceutical Sciences, IJPS |
| ISSN | 19050852 |
| Abstract | The aqueous extract of Bauhinia penicillilobaPierre ex Gagnep root, traditionally used as a tonic and appetizer, was investigated for immunomodulating, antioxidant and antimicrobial activities. The immunomodulating activity of aqueous extract was evaluated using mouse splenocytes proliferation by BrdU assay. The results showed that B. penicillilobahad an immunostimulating activity. The extract alone exhibited stimulating activity at the concentration range of 12.5-400 ?g/ml with the maximum activity at 200 ?g/ml (P.I. = 2.95; control = 1.00). In the presence of pokeweed mitogen (PWM), the extract significantly increased lymphocyte proliferation at concentration range of 12.5-100 ?g/ml with the strongest activity at 50 ?g/ml (P.I. = 5.68; control = 4.25). The effect of aqueous extract on phagocytic activity of mouseperitoneal macrophage was evaluated by NBT assay. The aqueous extract at the range of 100-400 ?g/ml gave non-significant stimulating effects. The concentration of extract exhibiting maximum activity was 400 ?g/ml (NBT reduction index = 1.14; control = 1.00). The effect of B. penicillilobaextract on cytokine production was determined by ELISA kits. The extract with concanavalin-A (ConA) augmented IFN-?production at concentration of 100 ?g/ml (1076.20 pg/ml; control = 981.20 pg/ml). But the extract alone showed inhibitory effects on IL-4 production. The aqueous extract of B. penicillilobaalso exhibited potent antioxidant activity based on DPPH and FRAP assay with the EC 50 and FRAP values of 11.34 ?g/ml and 2,159.72 ?M, respectively. The total phenolic compounds determined by Folin-Ciocalteau assay was 249.96 ?g/mg. For antimicrobial activity, B. penicillilobaaqueous extract was more active against S. aureusthan B. cereus, B. subtilisand S. sonnei with inhibition zone of 10.82, 8.36, 7.33 and 7.03 mm, respectively. |
