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Evaluation of KaryoLite BACs on Beads assay for preimplantation genetic testing for aneuploidy in blastocyst |
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| รหัสดีโอไอ | |
| Creator | Watcharapa Jaranasaksakul, Takol Chareonsirisuthigul, Sawat Tritruengtassana, Chatchai Treetampinich, Prapatsorn Areesirisuk, Rachanee Parinayok, Budsaba Rerkamnuaychoke |
| Title | Evaluation of KaryoLite BACs on Beads assay for preimplantation genetic testing for aneuploidy in blastocyst |
| Contributor | Watcharapa Jaranasaksakul, Takol Chareonsirisuthigul, Sawat Tritruengtassana, Chatchai Treetampinich, Prapatsorn Areesirisuk, Rachanee Parinayok, Budsaba Rerkamnuaychoke |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2564 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 14 |
| Journal No. | 1 |
| Page no. | 1-8 |
| Keyword | KaryoLiteBACs on Beads, PGT-A, blastocyst, trophectoderm, embryo |
| URL Website | https://li01.tci-thaijo.org/index.php/gst/issue/view/17009 |
| Website title | https://li01.tci-thaijo.org/index.php/gst/article/view/245400 |
| ISSN | 24655198 |
| Abstract | Preimplantation genetic testing for aneuploidy (PGT-A) is used to screen chromosomal abnormalities in embryos. Currently, a new molecular karyotyping platform, KaryoLite bacterial artificial chromosomes (BAC)son Beads (KL-BoBs) assay was developed to detect aneusomy for any chromosomes. Here, we present an evaluation of KL-BoBs for a routine diagnostic test in a clinical setting for PGT-Aby comparison with array-based comparative genomic hybridization (aCGH)result. A total of 39 samples from 14 frozen blastocysts were obtained from 3 patients. The frozen blastocysts were studied in the first biopsy, and aCGH was tested parallelly to detect chromosomal abnormalities in biopsied trophectoderm (TE). Consistencyof the results obtained from biopsied inner cell mass (ICM) and TE was 98.2%. The biopsied TE in human blastocysts can predict the chromosome information in ICM. We found that 36.36% of TE from the same abnormal blastocysts had mosaicism. Accordingto thelimitation of KL-BoBs, polyploidy and structural abnormalities were unable to be detected. The results from KL-BoBs had concordance with those from aCGH technique to detect chromosomal abnormalities for PGT-A. Consistency of biopsied ICM and TE was 98.2%,which indicated that biopsied TE in human blastocystscould predict chromosome information in ICM. |
