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Brassinosteroids promote AUX/IAA transcriptional repressors and inhibit adventitious root formation in mung bean |
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| รหัสดีโอไอ | |
| Creator | Ananya Romyanon, Juthamas Chaiwanon |
| Title | Brassinosteroids promote AUX/IAA transcriptional repressors and inhibit adventitious root formation in mung bean |
| Contributor | Ananya Romyanon, Juthamas Chaiwanon |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2563 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 13 |
| Journal No. | 1 |
| Page no. | 12-18 |
| Keyword | adventitious root formation, brassinosteroid, auxin |
| URL Website | https://li01.tci-thaijo.org/index.php/gst/issue/view/16612 |
| Website title | https://li01.tci-thaijo.org/index.php/gst/article/view/241214 |
| ISSN | 24655198 |
| Abstract | Adventitious root (AR) formation is important in asexual propagation of horticultural and forestry crops. It is induced by wounding and controlled by several phytohormones, including auxin. Brassinosteroids (BR) are plant steroid hormones that regulate several aspects of plant growth and development and interact with auxin. Here, we investigated roles of BR in AR formation in mung bean hypocotyl cuttings by dipping the hypocotyl base of the explants in various concentrations of BR (1 nM, 10 nM, 0.1 ?M, 1 ?M and 10 ?M 24-epi-castasterone). Our results demonstrated that BR treatments suppressed AR formation in a dose-dependent manner. Expression analysis of genes involved in auxin signaling and transport showed that BR up-regulatedseveral genes encodingAUX/IAA transcriptional repressors, but not an auxin transporter PIN1. Analyses of activities of polyphenol oxidase (PPO), which plays roles in root primordia formation, suggested that BR may inhibit AR formation partly by reducing PPO activity in the hypocotyl cuttings. Our findings revealed roles of BR in promoting transcription of negative regulators of the auxin signaling pathway, thus blocking auxin-mediated AR formation. |
