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Differential expression of Ran-binding protein 2 (RanBP2) and Targeting Protein for Xklp2 (TPX2) in head and neck squamous cell carcinoma cells |
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| รหัสดีโอไอ | |
| Creator | Ekarat Hitakomate, Sittichai Koontongkaew |
| Title | Differential expression of Ran-binding protein 2 (RanBP2) and Targeting Protein for Xklp2 (TPX2) in head and neck squamous cell carcinoma cells |
| Contributor | Ekarat Hitakomate, Sittichai Koontongkaew |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2562 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 12 |
| Journal No. | 2&3 |
| Page no. | 54-62 |
| Keyword | head and neck cancer, RanGTPase, RanBP2, TPX2 |
| URL Website | https://li01.tci-thaijo.org/index.php/gst/issue/view/15103 |
| Website title | https://li01.tci-thaijo.org/index.php/gst/article/view/144160 |
| ISSN | 24655198 |
| Abstract | RanGTPase is overexpressed in various human cancer cell lines. RanGTPase-related genes are essential for viability of head and neck cancer cell lines. However, it is currently unclear whether RanGTPase play a role in metastasis of head and neck cancer. This study aimed to examine mRNA and protein expressions of RanGTPase in primary and metastatic head and neck cancer cell lines using real-time PCR and Western blot analyses. Three pairs of head and neck cancer cell lines were used. HN18, HN30 and HN4 were derived from primary lesions, whereas HN17, HN31 and HN12 were derived from metastasis lesions from to same patients, respectively. The results were compared to those obtained from HaCat, an immortalized non-tumorigenic human keratinocyte cell line. Cells were cultured and total RNA was extracted and converted to complementary DNA. Expression at the mRNA level of Ran, KPNB1, RanBP2, and TPX2 was monitored using real-time PCR. GAPDH was used as an internal control. Western blotting was also performed to examine the protein expression of Ran, Importin-beta, RanBP2 and TPX2. Real-time PCR data showed that mRNA expression of Ran, KPNB1, RanBP2 and TPX2 in primary and metastatic head and neck cancer cell lines was significantly different from that of HaCat at p value of 0.0079, 0.0005, 0.0074 and 0.0039, respectively. Furthermore, expression of mRNA transcripts of Ran and TPX2 in the metastatic head and neck cancer cell lines was higher than that of primary cancer cells. However, protein expression of RanBP2 and TPX2 was found to be higher in the metastatic cancer cells compared to their primary counterparts. These findings suggest that expressions of mRNA transcripts and proteins of RanGTPase network were significantly different from those of normal cells. Particularly, RanBP2 and TPX2 expressions was elevated in the metastatic head and neck cancer cell lines. Further studies are required to understand its functional role in metastatic head and neck cancer. |
