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Construction of Recombinant DNA Plasmid for Production 100 Base Pair DNA Ladder for Endless Usage Based on PCR Technique |
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| รหัสดีโอไอ | |
| Creator | Monthon Lertworapreecha, Jittakorn Thongnan |
| Title | Construction of Recombinant DNA Plasmid for Production 100 Base Pair DNA Ladder for Endless Usage Based on PCR Technique |
| Contributor | Monthon Lertworapreecha, Jittakorn Thongnan |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2561 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 11 |
| Journal No. | 1&2 |
| Page no. | 22-25 |
| Keyword | DNA ladder, synthetic DNA template, polymerase chain reaction |
| URL Website | https://www.tci-thaijo.org/index.php/gst/issue/view/12281 |
| Website title | https://www.tci-thaijo.org/index.php/gst/article/view/90676 |
| ISSN | 24655198 |
| Abstract | The standard 100 base pairs (bp) DNA ladder is an essential molecular marker in molecular biology laboratory. This standard DNA marker is normally used to determine the size of the fragments DNA, plasmid and PCR products for gel electrophoresis. Since the standard DNA ladder is one of the major costs of molecular biological research and teaching, therefore, the unlimited in-house production of this DNA ladder could be the effective cost reduction strategy in laboratories. Thus, the purpose of this study is to prepare a DNA ladder template by constructing plasmid DNA inserted with a synthetic 1,500 bp polynucleotides fragment. By using PCR technique, our results showed that the PCR technique able to amplify 11 fragments ranging from 100-1,000 bp and 1,500 bp with high qualification, accuracy in size. Finally, purification of the merged altogether of PCR products showed high quality and quantity of each ladder. Our procedure of in-house for production of 100 bp DNA could be simple, inexpensive, time saving and unlimited production. |
