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The polymorphism analysis of gene resistance to avian influenza virus (Mx gene) in KU-Phuphan black-bone chicken |
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| รหัสดีโอไอ | |
| Creator | Piyamas Phongkaew, Panuwat Khumpeerawat |
| Title | The polymorphism analysis of gene resistance to avian influenza virus (Mx gene) in KU-Phuphan black-bone chicken |
| Contributor | Piyamas Phongkaew, Panuwat Khumpeerawat |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2560 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 10 |
| Journal No. | 3 |
| Page no. | 62-69 |
| Keyword | antiviral gene, Mx gene, PCRRFLP, SNP |
| URL Website | https://www.tci-thaijo.org/index.php/gst/issue/view/9399 |
| Website title | https://www.tci-thaijo.org/index.php/gst/article/view/72572 |
| ISSN | 24655198 |
| Abstract | The Mx gene is an antiviral gene used to determine resistance or susceptibility to different types of viruses, including the Avian Influenza virus. Three populations of KUPhuphan blackbone chickens (KP), crossbreed broiler (BR) and layer (LA) chicken were examined resistant and sensitive genotypes of the Mx gene using PCRRFLP. DNA amplification results showed that the Mx gene was found in a 100 bp fragment. The Mx resistant genotype AA was found in both KP and BR, but not the LA population. The frequencies of allele A were 0.319, 0.443, and 0.550 for KP, BR and LA populations, respectively. The frequencies of allele G were 0.681, 0.557 and 0.450 for KP, BR and LA populations, respectively. While KP was in the Hardy-Weinberg equilibrium, BR and LA were not (P<0.01). Sequence analysis of homozygous resistant and sensitive genotype confirmed the single nucleotide substitution (SNP) causes a single amino acid substitution from Serine (Ser) to Asparagine (Asn) at position 631. It is important for the poultry industry to protect their birds from pathogenic infection, which correlated to antiviral performance. Therefore, the polymorphism in the Mx gene is important for the KP population to protect their chickens from pathogenic infection. Further studies are needed with regard to the genetic resistance to avian influenza in different populations with various domestication background and selection history. |
