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Expression Analysis of Rice Polygalacturonase cDNA Responding to Brown Planthopper [Nilaparvata lugens (Stal)] |
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| รหัสดีโอไอ | |
| Creator | Sugunya Suebsan |
| Title | Expression Analysis of Rice Polygalacturonase cDNA Responding to Brown Planthopper [Nilaparvata lugens (Stal)] |
| Contributor | Sugunya Suebsan, Supranee Sitthiphrom, Kanta Sangwijit, Mondhon Sanguansermsri, and Somboon Anuntalabhochai |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2560 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 10 |
| Journal No. | 1&2 |
| Page no. | 13-20 |
| Keyword | polygalacturonase, Oryza sativa cv. KDML105, insect herbivore, ethylene, cytokinin |
| URL Website | https://www.tci-thaijo.org/index.php/gst/issue/view/7723 |
| Website title | https://www.tci-thaijo.org/index.php/gst/article/view/75562 |
| ISSN | 24655198 |
| Abstract | A cDNA (OsKPG) encoding polygalacturonase (PG) from rice (Oryza sativa cv. KDML105) was cloned and sequenced. The cDNA full length was 1,103 bp and carried 277 deduced amino acids with 4 highly conserved domains among PG family. The expression of OsKPG was investigated under brown planthopper [Nilaparvata lugens (St?l)] attack. The transcription levels of OsKPG under different phytohormone applications including ethylene, abscisic acid (ABA), 6-benzyladenine (6-BA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) were also analyzed in leaves. Semi-quantitative RT-PCR revealed that the expression of OsKPG was high upon the brown planthopper attack, or under the application of ethylene or 6-BA treatments. This is the first evidence demonstrating that OsKPG may play a role in response against insect herbivore infestation through ethylene and cytokinin signaling pathways in rice. |
