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Estrogen activates Cyclooxygenase-2 and CD44 expressions and promotes cholangiocarcinoma cell invasion |
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| รหัสดีโอไอ | |
| Creator | Nuannapa In-chon, Chanitra Thuwajit, Peti Thuwajit |
| Title | Estrogen activates Cyclooxygenase-2 and CD44 expressions and promotes cholangiocarcinoma cell invasion |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2559 |
| Journal Title | Genomics and Genetics |
| Journal Vol. | 9 |
| Journal No. | 2&3 |
| Page no. | 95-103 |
| Keyword | cholangiocarcinoma, invasion, estrogen, cyclooxygenase-2, NS-398 |
| ISSN | 24655198 |
| Abstract | Cholangiocarcinoma (CCA) is one of the worst prognosis cancers. CCA usually presents in advanced stage according to its high metastatic property. Until present, the factors involving CCA metastasis are still unclear. Estrogen has been revealed as a stimulator of CCA invasiveness. Cyclooxygenase-2 (COX-2), an estrogen targeted gene, associates with cancer invasion and metastasis. This study aims to investigate the role of COX-2 in estrogen-stimulated invasion of CCA cells. KKU-100 and KKU-M213 CCA cell lines were treated with 17?-estradiol (E2), as active form of estrogen, and NS-398, a selective COX-2 inhibitor, and the invasive property of cancer cells was measured as well as the expression levels of some metastatic genes. The results indicated that E2 could stimulate CCA cell invasion and this process was inhibited by NS-398. In addition, in E2-stimulated CCA cells, the levels of COX-2 and CD44 were increased. These results indicate the impact of COX-2-mediated CD44 expression in E2-driven CCA invasion. The application of using COX-2 inhibitor to attenuate E2-stimulated invasion of CCA is of great interest to be proposed as an effective management of the disease. |
