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Biosynthesis ofAscorbic Acid by Aspergillus Flavusand Aspergillus Tamarii Immobilized in Afzelia AfricanaMatrix |
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| รหัสดีโอไอ | |
| Creator | TemitopeBanjo, SarafadeenKareem, Temitope Popoola and Oluseyi Akinloye |
| Title | Biosynthesis ofAscorbic Acid by Aspergillus Flavusand Aspergillus Tamarii Immobilized in Afzelia AfricanaMatrix |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2561 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 6 |
| Journal No. | 1 |
| Page no. | 39-52 |
| Keyword | Ascorbic acid, Immobilization, Afzelia Africana, Matrix, Aspergillus flavus |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/95431 |
| ISSN | 2286-8615 |
| Abstract | A novel matrix for the immobilization of ascorbic acidproduced by Aspergillus flavusandAspergillus tamariiwas reported. Spores of A. flavusand A. tamariiwere immobilized on Afzelia africana matrix cross-linked with glutaraldehyde (2.5%) and the effects of Afzelia africanagel concentration (913%), spore load (100500 mg/100ml), bead size (27 mm) and beadnumber (210) on ascorbic acid yield were determined.Theimmobilized fungiwere cultured in a liquid fermentation medium containing BSG (0.6% w/v) for ascorbic acid productionfor 144h. The ascorbic acid produced was quantified titrimetrically. Thestatistical analysis of theeffects of gel concentration, spore load and bead size on ascorbic acid production showed no significantdifference at p>0.05. However, there was significant difference in the effect of bead number on ascorbic acid production at p<0.05. Ascorbic acid yield of 8.5 g/L and 7.5 g/L was produced byAspergillus tamariiand Aspergillus flavusrespectively using 9 beads at 96 h of fermentation. The immobilized Aspergillustamariiand Aspergillusflavusretained activities of 72% and70% respectively after fiverepeated cycle and also exhibited increased activities over the free cells. This study shows the potential of Afzelia africanaas a novel matrixfor enhanced ascorbic acid production. |
