|
Utilization of waste glycerol as a carbon source for Pichia pastoriscultivation |
|---|---|
| รหัสดีโอไอ | |
| Creator | Nongnuch Singsun, Chartchai Kanongnuch, Noppol Leksawasdi and Ronachai Pratanaphon |
| Title | Utilization of waste glycerol as a carbon source for Pichia pastoriscultivation |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2559 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Page no. | 41-51 |
| Keyword | Waste glycerol, Biodiesel, Pichia pastoris |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/78282 |
| ISSN | 2286-8615 |
| Abstract | Waste glycerol is a by-product of thebiodiesel production process.Therefore, waste glycerol price is very inexpensive. The feasibility of cultivating Pichia pastorisusing waste glycerol as a carbon source was investigated. In this study, the composition of waste glycerol from 2 sources, E-ester company, Chiang Rai and Maechampork cracker community enterprise, Chiang Mai was analyzed. The average glycerol content was 27.70% and 29.82%, respectively. The simple methods to pretreat the waste glycerol were employed. Then the effect of treated and untreated waste glycerol on P. pastorisgrowth compared with pure glycerol was investigated. The suitable concentration of waste glycerol for P. pastoriscultivation was also assessed. Media containing 1, 2 and 5% (v/v) waste glycerol wereused to cultivate P. pastoris. The highest dry cell weight was achieved using 5% untreated waste glycerol. The maximum dry cell weight using waste glycerol from E-ester company, Chiang Rai and Maecham pork cracker community enterprise, Chiang Mai were 11.47 and 14.38 g/L, respectively. The high densityof biomassobtained from fermentationin a5-L bioreactor by using waste glycerol as a carbon source without any negative effect. This study revealed that waste glycerol from E-ester company, Chiang Rai and Maecham pork cracker community enterprise, ChiangMai can be directly used as a carbon source for P. pastoriscultivation. |
