|
Isolation and screening of protease producing halotolerant bacteria from fermented freshwater fishes |
|---|---|
| รหัสดีโอไอ | |
| Creator | Viraxay Bandavong, Kridsada Unban, Apinan Kanpiengjai and Chartchai Khanongnuch |
| Title | Isolation and screening of protease producing halotolerant bacteria from fermented freshwater fishes |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2559 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Page no. | 1-11 |
| Keyword | Halophiles, Protease, Siamese Mud Carp, Isolation, Kushneriasp. |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/78274 |
| ISSN | 2286-8615 |
| Abstract | A total of 45 halotolerant bacterial isolates were isolated from 38 samples of fermented freshwater fishes using modified MRS agar containing 20% (w/v) NaCl. Screening for protease production on modified MRS agar supplemented with 20% (w/v) NaCl and 0.5% (w/v) casein found that only 8 bacterial isolates formedclear zone and the isolates VK31 formedthe widest clear zone following by isolate VK20. Confirmation of protease production in liquid culture found that the enzyme activitiesproduced by both isolates werefound 4.41?0.02 and 3.35?0.04 U/mL, respectively. Based on the 16S DNA nucleotide sequence analysis, the isolate VK20 and VK31 were identified to be Virgibacillus halodenitrificans VK20 and Kushneria avicenniaeVK31, respectively. Time course of protease production at 37oC for 3 days showed the maximum protease activity of 5.42?0.02 and 4.19?0.03 U/mL by V. halodenitrificans VK20 and K. avicenniaeVK31, respectively. |
