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Removal of allergenic protein in natural rubber latex using protease from Bacillussp. |
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| รหัสดีโอไอ | |
| Creator | Suttiporn Nanti, Pairote Wongputtisin, Chotipa Sakulsingharoj, Augchararat Klongklaew and Niorn Chomsri |
| Title | Removal of allergenic protein in natural rubber latex using protease from Bacillussp. |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2557 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 2 |
| Journal No. | 3 |
| Page no. | 216-223 |
| Keyword | Protease, Bacillus sp., Natural rubber latex, Allergenic proteins, Rubber elongation factor |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/78062 |
| ISSN | 2286-8615 |
| Abstract | Natural rubber latex (NRL) contains more than 200 kinds of protein. Among these, 13 proteins have beenregarded as an allergen that causes allergenicity to the user;especially healthcare workers. Thus, enzymatic degradation of these proteins using Bacillus sp. protease was introduced in this study instead of using chemical and physical method to reduce the allergenicity rick of NRL products.This study wasaimed to produce protease powder from Bacillus sp.and determine minimum protease activity for NRL allergenic protein degradation.For the practical procedure,Bacillus sp. was cultured in Nutrient broth at 37 oC, onrotary shaker at 150 rpm for24 hand subsequently centrifuged to remove cells. Crude protease was precipitated by ammonium sulfate (80% saturation) anddialyzed (12 kDacut off). Powder protease was prepared by freeze drying and yellowish powder of proteaseform Bacillus sp. was subsequently obtained. The remaining protease activity was 120,422.53Units/ gpowder, while specific activity was169.78 unit/mg proteins. Differentamount of protease (0 -500 unit) wereadded to fresh NRL serum and then incubated at 45oC for 6 hr. The remaining of allergenHevb1 (rubber elongation factor; REF) was analyzed using western blot technique.REF could not be detected in NRL serum when more than 300 units of protease were added. Consequently, protease from Bacillus sp. coulddegrade REF in NRL. |
