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Optimal medium and conditions for phytase productionby thermophilic bacterium,Anoxybacillus sp.MHW14 |
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| รหัสดีโอไอ | |
| Creator | Apinun Kanpiengjai, Kridsada Unban, Ronachai Pratanaphon and Chartchai Khanongnuch |
| Title | Optimal medium and conditions for phytase productionby thermophilic bacterium,Anoxybacillus sp.MHW14 |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2556 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 1 |
| Journal No. | 3 |
| Page no. | 172-189 |
| Keyword | phytase, thermophilic bacteria, Anoxybacillus, response surface |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/77388 |
| ISSN | 2286-8615 |
| Abstract | Phytase isoneof severalenzymes thatwidely used in feed industry.Feed enzymes areexpected to have high thermostability to withstand the high temperature during feed processing. The main purpose of this research was tofind out the cheap and optimal medium and condition for production of phytase from a new source of phytase producing bacterium,Anoxybacillussp. MHW14.The suitable sourcesof phytic acid, carbon and nitrogen source wereselected according to the highest enzyme activity. Rice bran, glucose and ammonium sulfatewerefound to be the proper componentsas mentioned, respectively. The least square linear regression according Plackett and Burman design revealed that rice bran and ammonium sulfate were significant positive factors (p<0.05)on phytase production. The quadratic model of central composite design generated reliable predicted equation formaximumphytase activity with optimum rice bran and ammonium sulfate levelsof 10.35%and 2.64%(w/v), respectively.The validation of105% was attained within 0.20U/mLat 12 h of cultivation at 45oC which confirmed the successful optimization.Moreover, Anoxybacillussp. MHW14 was capable of producing the enzyme in broad pH (6.0-9.0)at the optimum temperature (45oC) in optimized medium. The unique characters of the bacterium were interesting and it was the first report ofphytase from Anoxybacillussp. |
