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Genetic Differentiation through ISSR Marker in the Common Puddle Frog (Occidozyga lima) Affected by Heavy Metals from a Municipal Landfill |
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| รหัสดีโอไอ | |
| Creator | 1. Lertsak Suemram 2. Bundit Tengjaroensakul 3. Manop Sriuttha 4. Sutee Chowrong 5. Somsak Intamat 6. Lamyai Neeratanaphan |
| Title | Genetic Differentiation through ISSR Marker in the Common Puddle Frog (Occidozyga lima) Affected by Heavy Metals from a Municipal Landfill |
| Publisher | Thai Society of Higher Education Institutes on Environment |
| Publication Year | 2567 |
| Journal Title | EnvironmentAsia |
| Journal Vol. | 17 |
| Journal No. | 3 |
| Page no. | 63-74 |
| Keyword | Frog, Municipal landfill, Heavy metals, Genetic, Genotoxicity |
| URL Website | http://www.tshe.org/ea/index.html |
| Website title | EnvironmentAsia |
| ISSN | 1906-1714 |
| Abstract | The concentrations of arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb) and manganese(Mn) in water, sediment and Occidozyga lima tissues were investigated, as well as the geneticdifferentiation of O. lima from a municipal landfill and a reference area. The heavy metalconcentrations were analyzed using inductively coupled plasma optical emission spectrometry(ICP-OES). Inter simple sequence repeats (ISSR) with dendrogram construction and genomictemplate stability (GTS) were employed to determine genetic differentiation. The concentrationsof As (0.0135 ? 0.01 mg/L), Cr (0.0866 ? 0.08 mg/L), and Pb (0.0808 ? 0.09 mg/L)in the water from the municipal landfill exceeded the standards (0.01, 0.05, and 0.05 mg/L,respectively), and the As concentration (8.9930 ? 1.47 mg/kg) in the sediment from the municipallandfill exceeded the standard (3.9 mg/kg). The heavy metal concentrations in O. lima from themunicipal landfill and reference area were within the standards. Genetic differentiation of O. limawas determined using ISSR fingerprints from 12 successful ISSR primers that generated 535 totalbands for dendrogram construction. The dendrogram results separated O. lima into two clusterscorresponding to the two sample sites. The genetic differentiation ranges of O. lima from the municipallandfill and reference area were 40.74 - 58.62% and 80.70 - 93.10%, respectively. The O. lima in themunicipal landfill had heavy metal accumulation and a low %GTS. These results demonstrated thegenotoxicity of heavy metals to frog that live in polluted areas by monitoring contaminant exposure. |
